Ion Ampliseq Library Kit 2.0 Manual Dexterity

ion ampliseq library kit 2.0 manual dexterity

Next-generation sequencing of tyrosine kinase BMC Cancer

specimens, one AmpliSeq primer pool (per reaction), and AmpliSeq library kit 2.0. Amplification was carried out on a GeneAmp PCR System 9700 (ThermoFisher Scientific) for 21 cycles.



ion ampliseq library kit 2.0 manual dexterity

Ion AmpliSeq Library Kit 2.0 Thermo Fisher Scientific

2.2. Manual library building, template preparation using Ion Chef™ and sequencing using Ion PGM™ DNA libraries were constructed using the Ion AmpliSeq™ Library Kit 2.0 (Thermo Fisher Scientific) combined with the Precision ID Identity primer panel. PCR was performed using half of the volumes of the reagents mentioned in the manufacturer’s protocol. The PCR contained 2 μl of 5X Ion

ion ampliseq library kit 2.0 manual dexterity

Illumina Sequencing Quantitation using Droplet Digital PCR

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ion ampliseq library kit 2.0 manual dexterity

Ion AmpliSeq Library Kit 2 tools.thermofisher.com

The application of the latest generation forensic DNA assays, such as the Quantifiler® Trio DNA Quantification Kit and the HID-Ion AmpliSeq™ Identity Panel (Applied Biosystems®), individuated

Ion ampliseq library kit 2.0 manual dexterity
Comparison of manual and automated AmpliSeq workflows in
ion ampliseq library kit 2.0 manual dexterity

Molecular genetic studies on EGFR KRAS BRAF ALK PIK3CA

Smoothened (SMO) is a seven 2013). Briefly, starting from 10 ng of FFPE DNA, library preparation was performed using Ion Amplis Equation 2.0 kit and Ion Ampliseq Cancer Panel primers (Thermo Fisher). From the barcoded library, a manual emulsion polymerase chain reaction was performed, followed by sequencing of eight multiplexed samples using the Ion Personal Genome Machine on Ion …

ion ampliseq library kit 2.0 manual dexterity

Oncotarget Genomic alterations at the basis of treatment

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ion ampliseq library kit 2.0 manual dexterity

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ion ampliseq library kit 2.0 manual dexterity

Validation of an NGS Approach for Diagnostic BRCA1/BRCA2

"This kit is optimized for streamlined DNA library preparation for Ion Torrent from intact DNA, and the kit includes a master mix for enzyme-based DNA fragmentation. Adaptors and primers for singleplex libraries are supplied in the kits. For multiplexed libraries, the Ion XPressTM Barcode Adaptors from Life Technologies can be used."

ion ampliseq library kit 2.0 manual dexterity

Quality Controls for AmpliSeq Hotspot . . . . . . . . . 3

library construction using the Ion Ampliseq Library Kit 2.0 (Life Technologies). Following quantification, libraries were equalized at 100 pM, attached and amplified on Ion Sphere Particles. Sequencing was performed using PGM seq 200 kit v2 or PGM Hi-Q kit by pooling 6-8 libraries on an Ion 316 chip v2 and 10 - 11 libraries on an Ion 318 chip v2 according to manufacturer’s protocol. Aimed

ion ampliseq library kit 2.0 manual dexterity

Targeted Next Generation Sequencing as a Reliable

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ion ampliseq library kit 2.0 manual dexterity

Product selection guide Ion GeneStudio S5 Systems

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ion ampliseq library kit 2.0 manual dexterity

Decision Tree for the Ion PGM System biorigami

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ion ampliseq library kit 2.0 manual dexterity

HID SNP Genotyper Plugin User Guide (Pub no. MAN0010641

The Ion OneTouch 200 Template Kit version 2 DL and the Ion OneTouch ES Instrument were then used for emulsion PCR and enrichment, and the Ion PGM 200 Sequencing Kit, Ion 318 Chips, and the PGM sequencing platform for massive parallel sequencing, as recommended by the manufacturers' protocols without modification. Sequencing data were analyzed with Torrent Suite for mapping and IGV for

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